A temperature-tolerant CRISPR base editor mediates highly efficient and precise gene editing in Drosophila.
Doll RM, Boutros M, Port F. Science Advances. 2023 Sep;9(35):eadj1568.
Summary: This paper describes a highly efficient base editor that can be used to install stop codons or generate protein variants with remarkable efficiency.
Tissue-Specific CRISPR-Cas9 Screening in Drosophila.
Port F, Boutros M. Methods Mol Biol. 2022;2540:157-176.
Summary: Book chapter summarising our appraoch to tissue-specific CRISPR screening with the Cas9 nuclease system.
Multiplexed conditional genome editing with Cas12a in Drosophila.
Port F, Starostecka M, Boutros M. PNAS. 2020 Sep 15;117(37):22890-22899.
Summary: In this paper we describe a highly active Cas12a nuclease and an associated sgRNA vector (pCFD8).
A large-scale resource for tissue-specific CRISPR mutagenesis in Drosophila.
Port F, Strein C, Stricker M, Rauscher B, Heigwer F, Zhou J, Beyersdörffer C, Frei J, Hess A, Kern K, Lange L, Langner N, Malamud R, Pavlović B, Rädecke K, Schmitt L, Voos L, Valentini E, Boutros M. Elife. 2020 Feb 13;9:e53865.
Summary: Description and characterization of the HD-CFD library, a large-scale sgRNA collection of tissue-specific in vivo mutagenesis.
Creating Heritable Mutations in Drosophila with CRISPR-Cas9.
Port F, Bullock SL. Methods Mol Biol. 2016;1478:145-160.
Summary: Book chapter summarising our appraoch to germline editing with Cas9.
Augmenting CRISPR applications in Drosophila with tRNA-flanked sgRNAs.
Port F, Bullock SL. Nature Methods. 2016 Oct;13(10):852-4.
Summary: We introduce a system for sgRNA multiplexing in Drosophila and show that expressing nuclease and sgRNAs from conditional promoters results in a marked increase in tissue-specificity.
Systematic Evaluation of Drosophila CRISPR Tools Reveals Safe and Robust Alternatives to Autonomous Gene Drives in Basic Research.
Port F, Muschalik N, Bullock SL. G3 (Bethesda). 2015 May 20;5(7):1493-502.
Summary: Systemematic comparison of different CRISPR tools and protocols.
Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila.
Port F, Chen HM, Lee T, Bullock SL. PNAS. 2014 Jul 22;111(29):E2967-76.
Summary: Description of our first suite of sgRNA vectors and transgenic Cas9 lines.